Page 1
Principles of Pulse Oximetry
Technology:
The principle of pulse
oximetry is based on the red and infrared light
absorption characteristics of oxygenated and
deoxygenated hemoglobin. Oxygenated hemoglobin
absorbs more infrared light and allows more red
light to pass through. Deoxygenated (or reduced)
hemoglobin absorbs more red light and allows
more infrared light to pass through. Red light
is in the 600-750 nm wavelength light band.
Infrared light is in the 850-1000 nm wavelength
light band.

Pulse oximetry uses a light
emitter with red and infrared LEDs that shines
through a reasonably translucent site with good
blood flow. Typical adult/pediatric sites are
the finger, toe, pinna (top) or lobe of the ear.
Infant sites are the foot or palm of the hand
and the big toe or thumb. Opposite the emitter
is a photodetector that receives the light that
passes through the measuring site.

There are two methods of
sending light through the measuring site:
transmission and reflectance. In the
transmission method, as shown in the figure on
the previous page, the emitter and photodetector
are opposite of each other with the measuring
site in-between. The light can then pass through
the site. In the reflectance method, the emitter
and photodetector are next to each other on top
the measuring site. The light bounces from the
emitter to the detector across the site. The
transmission method is the most common type used
and for this discussion the transmission method
will be implied.
After the transmitted red (R)
and infrared (IR) signals pass through the
measuring site and are received at the
photodetector, the R/IR ratio is calculated. The
R/IR is compared to a "look-up" table (made up
of empirical formulas) that convert the ratio to
an SpO2 value. Most manufacturers
have their own look-up tables based on
calibration curves derived from healthy subjects
at various SpO2 levels. Typically a
R/IR ratio of 0.5 equates to approximately 100%
SpO2, a ratio of 1.0 to approximately
82% SpO2, while a ratio of 2.0
equates to 0% SpO2.
The major change that occurred
from the 8-wavelength Hewlett Packard oximeters
of the '70s to the oximeters of today was the
inclusion of arterial pulsation to differentiate
the light absorption in the measuring site due
to skin, tissue and venous blood from that of
arterial blood.

At the measuring site there
are constant light absorbers that are always
present. They are skin, tissue, venous blood,
and the arterial blood. However, with each heart
beat the heart contracts and there is a surge of
arterial blood, which momentarily increases
arterial blood volume across the measuring site.
This results in more light absorption during the
surge. If light signals received at the
photodetector are looked at 'as a waveform',
there should be peaks with each heartbeat and
troughs between heartbeats. If the light
absorption at the trough (which should include
all the constant absorbers) is subtracted from
the light absorption at the peak then, in
theory, the resultants are the absorption
characteristics due to added volume of blood
only; which is arterial. Since peaks occur with
each heartbeat or pulse, the term "pulse
oximetry" was coined. This solved many problems
inherent to oximetry measurements in the past
and is the method used today in conventional
pulse oximetry.
Still, conventional pulse
oximetry accuracy suffered greatly during motion
and low perfusion and made it difficult to
depend on when making medical decisions.
Arterial blood gas tests have been and continue
to be commonly used to supplement or validate
pulse oximeter readings. The advent of "Next
Generation" pulse oximetry technology has
demonstrated significant improvement in the
ability to read through motion and low
perfusion; thus making pulse oximetry more
dependable to base medical decisions on.
